|
KMID : 0361020080510050435
|
|
Korean Journal of Otolaryngology - Head and Neck Surgery
2008 Volume.51 No. 5 p.435 ~ p.440
|
|
|
Inhibitory Effect of 15-Deoxy-Delta12,14-Protaglandin J2 on the MUC8 Expression in the Human Airway Epithelial Cells
|
|
Woo Hyun-Jae
Bae Chang-Hoon
Song Si-Youn
Kim Yong-Dae
|
|
|
Abstract
|
|
|
Background & Objectives: Among the numerous mucin genes, MUC8 is regarded as one of the most important mucin genes which are related with upper airway disease such as a nasal polyp. 15-Deoxy-delta(12,14)-prostaglandin J(2)(15d-PGJ(2)), the most recently discovered prostaglandin, has been known to have multiple cellular functions, including anti-inflammatory and cytoprotective effects. However, the effect of 15d-PGJ(2) on mucin gene expression or mucin production has not yet been investigated. The purpose of this study was to determine the effect of 15d-PGJ(2) on interleukin-1beta (IL-1beta)-induced MUC8 gene expression and mucin secretion in the cultured NCI-H292 cells and human nasal polyp epithelial cells.
Subjects & Method: The MUC8 mRNA levels were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and MUC8 mucin levels were measured using an enzyme-linked immunosorbent assay (ELISA) in the cultured epithelial cells stimulated by IL-1beta. 15d-PGJ(2) was added 1 hour before stimulation.
Results: 15d-PGJ(2) attenuated the IL-1beta-induced MUC8 mRNA expression and mucin secretion with a dose-dependent pattern in both cultured NCI-H292 cells and human nasal polyp epithelial cells.
Conclusions: These results suggest that 15d-PGJ(2) may be considered as an effective agent for the control of airway mucus hypersecretion through the down-regulation of MUC8 gene.
|
|
|
KEYWORD
|
|
|
Nasal polyp, MUC8, Mucin, 15d-PGJ2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|